ParaBioSys - Parallel Biological Systems - MGH, HMS, BU
Signal Transduction
Host-Pathogen Interactions
Protein Networks/RNA Display
Macrophage Activation
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Bacterial Pathogens
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The Five Research Centers:

  1. Microarray and Sequencing Center

    Provide all synthetic oligonucleotides, DNA sequencing, cDNA library construction, and DNA array generation for Program investigators.

  2. Education and Training Center

    Coordinate education and training in all of the methods utilized by the components of the PGA. Will organize training courses for non-Program and other visiting scientists

  3. Proteomics and Molecular Biology Center

    Create the molecular biologic and proteomic tools necessary to conduct screens for protein-protein interactions leading to the identification of PDZ domain binding partners and kinase substrates. The development of improved methods for performing gene expression and interactive protein studies will be centered in this component.

  4. Human Tissue and Animal Model Center

    Provide animal models for all experiments at the MGH and will provide pathology and nucleic acid preparation services for the cystic fibrosis component at the Channing/Brigham and Women's Hospital.. Animal services include breeding, genotyping, and in vivo or in vitro characterization of cells or tissues (e.g., binding assays, Western blotting, Northern analysis, ELISAs). In vitro differentiation of embryonal stem cells to macrophages will be provided for studies of gene knock-outs that cause embryonic lethality (e.g., PPAR-g). Collection and preservation of human tissues for gene profiling will be performed by this component along with pathological characterization of tissue samples by light, confocal, and electron microscopy. Gene transduction using adenoviral vectors to express wild type or mutant forms of proteins in tissues or cultured cells will also be provided.

  5. Bioinformatics Center

    Provide all computational biology services to Program investigators including, but not limited to, definition and in silico characterization of gene families, software development for process automation of array generation, database management of all sequence information, analytical tools for deconstructing arrays and cluster analysis of gene networks, creation of user-friendly interfaces for Program investigators and for public access to the data generated.

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