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The Five Research Centers:
Microarray and Sequencing Center
Provide all synthetic oligonucleotides,
DNA sequencing, cDNA library construction, and DNA array generation
for Program investigators. Education and Training Center
Coordinate education and training in all of the methods utilized by
the components of the PGA. Will organize training courses for non-Program
and other visiting scientists Proteomics and Molecular Biology Center
Create the molecular biologic and proteomic tools necessary to conduct
screens for protein-protein interactions leading to the identification
of PDZ domain binding partners and kinase substrates. The development
of improved methods for performing gene expression and interactive protein
studies will be centered in this component. Human Tissue and Animal Model Center
Provide animal models for all experiments at the MGH and will provide
pathology and nucleic acid preparation services for the cystic fibrosis
component at the Channing/Brigham and Women's Hospital.. Animal services
include breeding, genotyping, and in vivo or in vitro characterization
of cells or tissues (e.g., binding assays, Western blotting, Northern
analysis, ELISAs). In vitro differentiation of embryonal stem cells
to macrophages will be provided for studies of gene knock-outs that
cause embryonic lethality (e.g., PPAR-g). Collection and preservation
of human tissues for gene profiling will be performed by this component
along with pathological characterization of tissue samples by light,
confocal, and electron microscopy. Gene transduction using adenoviral
vectors to express wild type or mutant forms of proteins in tissues
or cultured cells will also be provided. Bioinformatics Center
Provide all computational biology services to Program investigators
including, but not limited to, definition and in silico characterization
of gene families, software development for process automation of array
generation, database management of all sequence information, analytical
tools for deconstructing arrays and cluster analysis of gene networks,
creation of user-friendly interfaces for Program investigators and for
public access to the data generated.
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