Bacterial Pathogen ResourcesPA14 Genomic SequencePseudomonas aeruginosa is an important soil bacterium, with a complex metabolism capable of degrading polycyclic aromatic hydrocarbons, and producing interesting, biologically active secondary metabolites including quinolones, rhamnolipids, lectins, hydrogen cyanide, and phenazines. Production of these products is likely controled by complex regulatory networks making Pseudomonas aeruginosa adaptable both to free-living and pathogenic lifestyles. The complexity of its metabolism is reflected in a relatively large genome size (about 6.5 Mb). The sequence for P. aeruginosa strain PAO1 can be accessed here. We have sequenced the more virulent strain, PA14, as described here. The current assembly consists of a single 6.54 Mb contig, available for download. A draft version of the PA14 annotation will be posted here when available. PA14 Mutant LibraryOne of the major products of the MGH PGA program will be a library of transposon mutants in the genes of Pseudomonas aeruginosa. Our specific strategy is to randomly mutagenize P. aeruginosa strain PA14 with transposons TnphoA and Mariner, determine the sites of insertion of approximately 30,000 mutants, and from this collection, choose a subset corresponding to a single transposon insertion in each annotated non-essential gene. As part of our ongoing transposon mutant sequencing project, we are making available a searchable database of transposon mutants of non-essential PA14 genes. This tool provides an interface by which researchers can search the transposon insertion mutant libraries for mutants in a given gene. If desired, a comprehensive list of identified mutants can be downloaded. Mutants of interest from the library can be ordered through the on-line Mutant Request interface PowerPoint PresentationsThe following PowerPoint presentations are available for online viewing or download.
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