| Primer Pair Descriptions: |
| PrimerBank ID |
34328442a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
137 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
TCATACTTCGGTTGCATGAAGG |
22 |
60.4 |
31-52 |
| Reverse Primer |
AGACCTCTCGAACCTGCCC |
19 |
63.0 |
167-149 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atgaccatcc ttttccttac tatggttatt tcatacttcg gttgcatgaa ggcggcgccc
61 atgaaagaag taaacgtcca cggacaaggc aacttggcct acccaggtgt gcggacccat
121 gggactctgg agagcgtgaa tgggcccagg gcaggttcga gaggtctgac gacgacatca
181 ctggctgaca cttttgagca cgtcatcgaa gagctgctgg atgaggacca gaaggttcgg
241 cccaacgaag aaaaccataa ggacgcggac ttgtacactt cccgggtgat gctcagcagt
301 caagtgcctt tggagcctcc tctactcttt ctgctggagg aatacaaaaa ttacctggat
361 gccgcaaaca tgtctatgag ggttcggcgc cactccgacc ctgcccgccg tggggagctg
421 agcgtgtgtg acagtattag cgagtgggtc acagcggcag ataaaaagac tgcagtggac
481 atgtctggcg ggacggtcac agtcctagag aaagtcccgg tatccaaagg ccaactgaag
541 cagtatttct acgagaccaa gtgtaatccc atgggttaca ccaaggaagg ctgcaggggc
601 atagacaaaa ggcactggaa ctcgcaatgc cgaactaccc aatcgtatgt tcgggccctt
661 actatggata gcaaaaagag aattggctgg cgattcataa ggatagacac ttcctgtgta
721 tgtacactga ccattaaaag gggaagatag |
