| Primer Pair Descriptions: |
| PrimerBank ID |
31981525a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
74 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
TGAGCAACTATTCCAAACCAGC |
22 |
60.8 |
933-954 |
| Reverse Primer |
GCACGTAGTCTTCGATCACTATC |
23 |
60.3 |
1006-984 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atgctcttcc gagctgtgct gctctgcgct gccctggggc tcagccaggc agcaaatcct
61 tgctgttcca atccatgtca aaaccgtggg gaatgtatga gcacaggatt tgaccagtat
121 aagtgtgact gtacccggac tggattctat ggtgaaaact gtactacacc tgaatttctg
181 acaagaatca aattactgct gaagcccacc ccaaacacag tgcactacat cctgacccac
241 ttcaagggag tctggaacat tgtgaacaac atccccttcc tgcgaagttt aatcatgaaa
301 tatgtgctga catccagatc atatttgatt gacagtccac ctacttacaa tgtgcactat
361 ggttacaaaa gctgggaagc cttctccaac ctctcctact acaccagggc ccttcctccc
421 gtagcagatg actgcccaac tcccatgggt gtgaagggaa ataaggagct tcctgattca
481 aaagaagtgc tggaaaaggt tcttctacgg agagagttca tccctgaccc ccaaggctca
541 aatatgatgt ttgcattctt tgcccagcac ttcacccatc agtttttcaa gacagatcat
601 aagcgaggac ctgggttcac ccgaggactg ggccatggag tggacttaaa tcacatttat
661 ggtgaaactc tggacagaca acataaactg cgccttttca aggatggaaa attgaaatat
721 caggtcattg gtggagaggt gtatcccccc acagtcaaag acactcaggt agagatgatc
781 taccctcctc acatccctga gaacctgcag tttgctgtgg ggcaggaagt ctttggtctg
841 gtgcctggtc tgatgatgta tgccaccatc tggcttcggg agcacaacag agtgtgcgac
901 atactcaagc aggagcatcc tgagtggggt gatgagcaac tattccaaac cagcagactc
961 atactcatag gagagactat caagatagtg atcgaagact acgtgcaaca cctgagcggt
1021 taccacttca aactcaagtt tgacccagag ctccttttca accagcagtt ccagtatcag
1081 aaccgcattg cctctgaatt caacacactc tatcactggc accccctgct gcccgacacc
1141 ttcaacattg aagaccagga gtacagcttc aaacagtttc tctacaacaa ctccatcctc
1201 ctggaacatg gactcactca gtttgttgag tcattcacca gacagattgc tggccgggtt
1261 gctgggggaa gaaatgtgcc aattgctgta caagcagtgg caaaggcctc cattgaccag
1321 agcagagaga tgaaatacca gtctctcaat gagtaccgca aacgcttctc cctgaagccg
1381 tacacatcat ttgaagaact tacaggagag aaggaaatgg ctgcagaatt gaaagccctc
1441 tacagtgaca tcgatgtcat ggaactgtac cctgccctgc tggtggaaaa acctcgtcca
1501 gatgctatct ttggggagac catggtagag cttggagcac cattctcctt gaaaggactt
1561 atgggaaatc ccatctgttc tcctcaatac tggaagccga gcacctttgg aggcgaagtg
1621 ggttttaaga tcatcaatac tgcctcaatt cagtctctca tctgcaataa tgtgaagggg
1681 tgtcccttca cttctttcaa tgtgcaagat ccacagccta ccaaaacagc caccatcaat
1741 gcaagtgcct cccactccag actagatgac attaacccta cagtactaat caaaaggcgt
1801 tcaactgagc tgtaa |
