| Primer Pair Descriptions: |
| PrimerBank ID |
31981070a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
100 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
CACGGCGAGTTTGGTTCCTA |
20 |
62.1 |
49-68 |
| Reverse Primer |
CTTCTGGCTTTGGGCGTAGT |
20 |
62.2 |
148-129 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atggccccac caccactact gccagtggct gccagcactc caatcctgca cggcgagttt
61 ggttcctacc cggccaacgg cccacggttt gccctcaccc tcacaacaca agccctacac
121 atacagcgac tacgcccaaa gccagaagcc cggccccgag atggtctagt ctctctggat
181 gaggtctcgg gctgtggcac cctgcagagc cgtagccccg aggacactgc agcctacttc
241 tgcatctaca cctacccacg tggccgtcga gggggccggc gcagagctac gcggaccttc
301 cgggcggatg gggccaccac ttatgaggag aatcgtgcag aggcccagcg ctgggccact
361 gccctcacgt gtctcctccg aggagtgcct ctgtcagggg accaggaaat cacccctgaa
421 ttgctgcccc ggaagcccag gctgctcata ttggtcaatc cctttggggg gcggggcctg
481 gcctggcagc gctgtatgga ccacgtggtg ccaatgatct ctgaagctgg gctgtccttc
541 aacctcatac agacagaacg acagaaccat gcccgtgagc tggtgcaggg gttaagcctg
601 agtgagtggg aaggcattgt cactgtgtct ggagacgggc tgctttacga ggtgctgaat
661 gggctccttg atcggccaga ctgggaggat gccgtgcgga tgcccattgg tgtcctcccc
721 tgtggatcgg gcaatgcgct agctggggcg gtgaaccatc atggcgggtt tgagcaggtt
781 gtcggtgttg acctgttgct caactgctcg cttcttctct gccgtggtgg cagccatcct
841 ctggacctgc tctctgtgac gctagcctcg ggatcccgct gtttttcctt cctgtcagtg
901 gcctggggat tcttgtcaga tgtggacatt cacagtgagc gcttcagggc cctgggcagc
961 gctcgattca cactgggtgc agtgctaggc ctggcctcgt tgcataccta ccgtggacgc
1021 ctctcctacc tccccgctac cacagaacca gccttgccca tcccaggcca cagtctgcct
1081 cgagccaagt cagaactagt cttggctcca gccccagccc ccgccgccac ccactcgcct
1141 ctacatcgat ctgtgtctga cctgcccctg ccccttcccc agcctgcctt ggtctcccct
1201 ggctcccctg agcccctgcc tgacctgtcc ctcaatggtg gtggtccaga gctgactgga
1261 gactggggag gagctgggga tgcacctctg tccccagacc cactgctgcc ttcatccccc
1321 aacgctctca aaacagctca gctttcaccc atcgctgaag ggcccccaga aatgccagca
1381 tcttcggggt tcctgcctcc cacccacagt gccccagaag cctctacctg gggcccagtg
1441 gaccacctcc tccctcccct gggctctcca ctgccccaag actgggtgac aatagagggg
1501 gagtttgtac tcatgttggg catcttgccg agccacctct gcgcagacct gatggcagcc
1561 ccacatgcac gctttgatga tggcgttgtg cacctgtgtt gggtgcggag cggcatctca
1621 cgggctgcac ttctacgcat tttgctggcc atggagcatg gaaaccactt cagcctgggc
1681 tgcccccatc tgggctatgc tgcagcacgt gccttccgcc ttgaaccact cacgcctcgt
1741 ggcctgctca ctgtagatgg ggagttagtg gagtatgggc caatacaggc gcaggtgcac
1801 ccaggtctcg ccacgctgct cactgggcct gcaggtcaaa agccacaagc ctga |
