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The following primer pair is found for 260763909c1
| Gene Descriptions: |
| NCBI GeneID |
221150 |
| GenBank Accession |
NM_145061 |
| NCBI Protein Accession |
NP_659498 |
| Species |
Human |
| Coding DNA Length |
1239 |
| Gene Description |
Homo sapiens spindle and kinetochore associated complex subunit 3 (SKA3), transcript variant 1, mRNA. |
| Primer Pair Descriptions: |
| PrimerBank ID |
260763909c1 |
| Amplicon Size |
176 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
TACACGAGCAAGAAGCCATTAAC |
23 |
60.9 |
332-354 |
| Reverse Primer |
GGATACGATGTACCGCTCAAGT |
22 |
61.6 |
507-486 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atggacccta tccggagctt ctgcgggaag ctgcggtctc tggccagcac gctggactgc
61 gagacggccc ggctgcagcg agcgctggac ggagaggaaa gcgactttga agattatcca
121 atgagaattt tatatgacct tcattcagaa gttcagactc taaaggatga tgttaatatt
181 cttcttgata aagcaagatt ggaaaatcaa gaaggcattg atttcataaa ggcaacaaaa
241 gtactaatgg aaaaaaattc aatggatatt atgaaaataa gagagtattt ccagaagtat
301 ggatatagtc cacgtgtcaa gaaaaattca gtacacgagc aagaagccat taactctgac
361 ccagagttgt ctaattgtga aaattttcag aagactgatg tgaaagatga tctgtctgat
421 cctcctgttg caagcagttg tatttctgag aagtctccac gtagtccaca actttcagat
481 tttggacttg agcggtacat cgtatcccaa gttctaccaa accctccaca ggcagtgaac
541 aactataagg aagagcccgt aattgtaacc ccacctacca aacaatcact agtaaaagta
601 ctaaaaactc caaaatgtgc actaaaaatg gatgattttg agtgtgtaac tcctaaatta
661 gaacactttg gtatctctga atatactatg tgtttaaatg aagattacac aatgggactt
721 aaaaatgcga ggaataataa aagtgaggag gccatagata cagaatccag gctcaatgat
781 aatgtttttg ccactcccag ccccatcatc cagcagttgg aaaaaagtga tgccgaatat
841 accaactctc ctttggtacc tacattctgt actcctggtt tgaaaattcc atctacaaag
901 aacagcatag ctttggtatc cacaaattac ccattatcaa aaacaaatag ttcatcaaat
961 gatttggaag ttgaagatcg tacttcgttg gttttaaatt cagacacatg ctttgagaat
1021 ttaacagatc cctcttcacc tacgatttct tcttatgaga atctgctcag aacacctaca
1081 cctccggaag taactaaaat tccagaagat attctccagc ttttatcaaa atacaactca
1141 aacctagcta ctccaatagc aattaaagca gtgccaccca gtaaaaggtt ccttaaacat
1201 ggacagaaca tccgagatgt cagcaacaaa gaaaactga |
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