Figure 2


Validation of a "PA14-Specific" ORF by Proteomics. In PAO1, the gene in between lecB and amiR is PA3362 (top), a gene annotated as a hypothetical protein. In PA14, a different predicted ORF, 6327, lies between these two genes (bottom). ORF 6327 does NOT have a homolog in PAO1, and is 53% identical (76% similar) to a conserved hypothetical protein found in Pseudomonas putita. Preliminary proteomic analysis of PA14 has identified a peptide mapping to this ORF (but not to any other predicted ORF in PA14).
Figure 2 - Validation of a "PA14-Specific" ORF by Proteomics. In PAO1, the gene in between lecB and amiR is PA3362 (top), a gene annotated as a hypothetical protein. In PA14, a different predicted ORF, 6327, lies between these two genes (bottom). ORF 6327 does NOT have a homolog in PAO1, and is 53% identical (76% similar) to a conserved hypothetical protein found in Pseudomonas putita. Preliminary proteomic analysis of PA14 has identified a peptide mapping to this ORF (but not to any other predicted ORF in PA14).