Figure 7


Tracking, Sorting and Analyzing Mutants: PA-TIMDB: Automation at several steps to facilitates the construction of the library, including the use of a colony picking robot, organization of library contents via barcodes, and the incorporation of a liquid handling robot to facilitate library replication, PCR setup, PCR, PCR cleanup and sequencing. PA-TIMDB tracks the mutants and PCR reactions during each processing stage. PA-TIMDB BLASTs the genomic sequences obtained for each mutant to reveal the disrupted gene for a given mutant.
Figure 7 - Tracking, Sorting and Analyzing Mutants: PA-TIMDB: Automation at several steps to facilitates the construction of the library, including the use of a colony picking robot, organization of library contents via barcodes, and the incorporation of a liquid handling robot to facilitate library replication, PCR setup, PCR, PCR cleanup and sequencing. PA-TIMDB tracks the mutants and PCR reactions during each processing stage. PA-TIMDB BLASTs the genomic sequences obtained for each mutant to reveal the disrupted gene for a given mutant.