The MGH-PGA Proteomic Database is based on NCBI's protein database, with the additional information about coding DNA sequences. For more detailed information about your protein, please refer to NCBI's protein search.
Our database was constructed by parsing the human and mouse GenPept release from NCBI. Very short peptide sequences (< 23 a.a.) were excluded from our database. Most immunoglobulin and T cell receptor variable region genes were also excluded to produce a better non-redundant database. The corresponding DNA coding regions were then retrieved from GenBank via the CDS feature and were included in our database.
With our database, you can do:
Database search for GenBank gene accession number, protein ID, GI, keyword, DNA sequence or peptide sequence One of the best features of our database is that you could easily identify the corresponding DNA coding sequences by searching for peptide sequences.
Batch Sequence Retrieval and Primer Design Once you have a list of peptide sequences in FASTA format, you may upload the file and get the corresponding DNA coding sequences and other GenBank features. 5' and 3' primers will also be designed for PCR of the DNA coding regions.
Primer Design For an input DNA sequence, both 5' and 3' primers are designed with a specified melting temperature (Tm).