Search for PCR Primers
You can blast your sequence against the primerbank sequence DB here.
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PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time PCR). PrimerBank contains over 306,800 primers covering most known human and mouse genes. There are several ways to search for primers: GenBank Accession, NCBI protein accession, NCBI Gene ID, Gene SymbolNew!, PrimerBank ID or Keyword (gene description) or you can blast your gene sequence against the primerbank Sequence DBNew!.
The primer design algorithm has been extensively tested by real-time PCR experiments for PCR specificity and efficiency. We have tested 26,855 primer pairs that correspond to 27,681 mouse genes by Real Time PCR followed by agarose gel electrophoresis and sequencing of the PCR products. The design success rate is 82.6% (22,187 successful primer pairs) based on agarose gel electrophoresis.
All experimental validation data for mouse primers are available from PrimerBank. In order to view, please follow the appropriate links seen on the primer information page.
Publication:
Xiaowei Wang, Athanasia Spandidos, Huajun Wang and Brian Seed: PrimerBank: a PCR primer database for quantitative gene expression analysis, 2012 update.
Nucl. Acids Res. (2012) 40 (D1): D1144-9.
Athanasia Spandidos, Xiaowei Wang, Huajun Wang and Brian Seed: PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification
Nucleic Acids Research 2010 38:D792-9.
Athanasia Spandidos, Xiaowei Wang, Huajun Wang, Stefan Dragnev, Tara Thurber and Brian Seed: A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance.
BMC Genomics 2008, 9:633
Xiaowei Wang and Brian Seed: A PCR primer bank for quantitative gene expression analysis.
Nucleic Acids Research 2003 31(24): e154; pp.1-8.
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