| Primer Pair Descriptions: |
| PrimerBank ID |
8393313a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
128 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
ACCATCAGCATAGGTGGACTT |
21 |
60.6 |
79-99 |
| Reverse Primer |
ACGTGGTAGTTCAAATGGAAGG |
22 |
60.2 |
206-185 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atggggcaaa gcgtgctccg ggcggtcttc tttttagtcc tggggctttt gggtcattct
61 cacggaggat tccccaacac catcagcata ggtggacttt tcatgagaaa cacggtacag
121 gagcacagtg ctttccgctt tgctgtgcag ttatacaaca ccaaccagaa caccactgag
181 aagcccttcc atttgaacta ccacgtagac cacttggatt cctccaatag tttttctgtg
241 actaatgctt tctgctccca gttctccaga ggggtgtatg ctatctttgg attctatgac
301 cagatgtcaa tgaacacact gacctccttc tgtggggccc tgcatacatc tttcgtcaca
361 cctagctttc ccactgatgc cgatgtgcag tttgtcatcc agatgcgccc agccttaaag
421 ggtgccattc tgagtcttct gggttactac aagtgggaga agtttgtgta cctctatgac
481 acagaacgag ggttttccat cctgcaagca attatggaag cagcagtgca aaacaactgg
541 caagtgacag caaggtctgt gggaaacata aaggacatcc aggaattcag acgcatcatt
601 gaagaaatgg acagaaggca ggaaaaacga tacttgattg actgtgaagt cgaaaggatt
661 aacacaattt tggaacaggt tgtgatcctg gggaaacatt caagaggtta tcactacatg
721 cttgctaacc tgggttttac tgacattgta ctggaaagag tcatgcatgg gggagccaac
781 attacaggtt tccagattgt caacaatgaa aacccaatgg tccagcaatt catacagcgc
841 tgggtgagac tggatgaaag ggaattccct gaagccaaga atgcaccact aaagtataca
901 tctgcactga cacacgacgc aatactggtc atagcagaag ccttccgata cctgaggagg
961 cagcgagtgg atgtatcccg cagaggcagt gctggagact gcttagcaaa tcctgctgtg
1021 ccctggagtc aaggaattga tattgagaga gctctgaaaa tggtgcaagt acaaggaatg
1081 actggaaaca tccaatttga cacttatgga cgtaggacaa attataccat tgatgtatat
1141 gaaatgaaag tctcaggctc tcgaaaagct ggttactgga atgagtatga aaggtttgtg
1201 cccttctcag atcaacaaat cagcaatgac agctcatcct ccgagaacag gaccatcgta
1261 gtgactacca ttctggaatc accatatgta atgtataaaa agaatcatga gcaactggaa
1321 ggaaatgagc gctatgaagg ctattgtgtc gatttagcct atgaaatagc caaacacgta
1381 aggatcaaat acaaattgtc cattgtcggt gatgggaaat atggcgcaag ggatccagag
1441 actaaaatat ggaatggcat ggttggggaa cttgtctatg gaagagctga tatagctgtt
1501 gctccactca ctataacatt ggtccgtgaa gaagtcatag atttttcaaa gccatttatg
1561 agcctgggga tctccatcat gataaagaag cctcagaaat caaagccagg cgtattttca
1621 ttcctggatc ctttagctta tgaaatctgg atgtgcattg tcttcgctta cattggagtc
1681 agtgtagttc tcttcctagt cagcagattt agcccttatg agtggcactt ggaagacaac
1741 aatgaagaac ctcgtgaccc acaaagccct cctgatcctc ccaatgaatt tggaatattt
1801 aacagtcttt ggttttcctt gggtgctttt atgcagcaag gatgtgatat ttctccaaga
1861 tcactttctg ggcgcattgt tggaggggtt tggtggttct tcaccctgat cataatctct
1921 tcctacactg caaaccttgc tgctttcctg actgtggaga ggatggtgtc ccccatagag
1981 agcgctgaag atttagccaa gcagactgaa attgcatacg ggaccctgga ctctggttca
2041 acaaaagaat ttttcagaag atccaaaatt gctgtgtatg agaaaatgtg gtcttacatg
2101 aaatccgcag agccatctgt gtttaccaaa acaacagctg atggggtagc ccgagttcgg
2161 aagtccaagg gaaagttcgc cttcctgctg gagtcaacca tgaatgagta cattgagcag
2221 agaaagccgt gtgatacgat gaaagttggt ggaaatctgg attccaaagg ctatggtgtg
2281 gcaaccccta aaggctcagc attaggaacg cctgtaaacc ttgcagtatt gaaactcagt
2341 gaacaaggca tcttagacaa gctgaaaaac aaatggtggt acgataaggg ggaatgtgga
2401 gccaaggact ccgggagtaa ggacaagacc agtgctctaa gcctgagcaa tgtggcaggc
2461 gtgttctata tacttgtcgg aggtctgggg ctggccatga tggtggcttt gatagaattc
2521 tgttacaaat cacgggcaga gtccaaacgc atgaaactca caaagaacac ccaaaacttt
2581 aagcctgctc ctgccaccaa cactcagaat tacgctacat acagagaagg ctacaacgtg
2641 tatggaacag aaagtgttaa gatctag |
