| Primer Pair Descriptions: |
| PrimerBank ID |
6754404a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
162 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
GAAACGCCCTTCTATGGCGA |
20 |
62.3 |
4-23 |
| Reverse Primer |
CAGCGCGTCTTTCTTCAGC |
19 |
61.7 |
165-147 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atggaaacgc ccttctatgg cgaggaggcg ctgagcggcc tggctgcggg tgcgtcgagc
61 gtcgctggtg ctactggggc ccccggcggt ggtggcttcg cgcccccggg ccgcgctttc
121 cccggggcgc ccccgacgag cagcatgctg aagaaagacg cgctgacgct cagcctggcg
181 gagcagggag cggcgggatt gaaaccaggg tcggccactg caccttctgc gctgcgcccc
241 gacggcgccc ccgacgggct gctggcttcg ccggatcttg ggctgctcaa actcgcgtcg
301 ccggagctgg agaggctgat catccagtcc aacgggctgg tgaccactac cccgaccagt
361 acgcagttcc tctacccgaa ggtggcagct agcgaggagc aggagttcgc cgaaggcttc
421 gtcaaggcgc tggaggacct gcacaagcaa agccagctgg gtgcggccac cgcggccacc
481 tcaggggctc ccgcgcctcc cgcgcccgcc gacctggccg ccacccccgg ggccacggag
541 accccggtct acgccaacct gagcagtttc gcgggtggcg ccgggccccc tgggggcgcg
601 gccaccgtgg ctttcgccgc ggagccagtg cccttcccgc cgcccccggg cgcgctgggg
661 ccgccgccac ctccgcatcc accgcgcctg gccgcgctca aggacgagcc gcagaccgtg
721 ccggacgtgc cgagcttcgg cgacagccct ccgctgtcgc ccatcgacat ggacacgcaa
781 gaacgcatca aggcggagcg caagaggctg cgcaaccgca tcgccgcctc caaatgccgc
841 aagcgcaagc tggagcgtat ctcgcgcctg gaggagaaag tcaagaccct caaaagccag
901 aacaccgagc tggcgtccac cgccagcctg ctgcgcgagc aggtggcgca gctcaaacag
961 aaagtcctca gccacgtcaa cagcggctgc cagctgctgc cccagcacca ggtcccggcg
1021 tactga |
