| Primer Pair Descriptions: |
| PrimerBank ID |
6754328a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
63 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
GCACCTTACACCTACCAGAGT |
21 |
60.9 |
346-366 |
| Reverse Primer |
AAACTTCTGCCTGACGAGCTT |
21 |
62 |
408-388 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atggccaaag ttcctgactt gtttgaagac ctaaagaact gttacagtga aaacgaagac
61 tacagttctg ccattgacca tctctctctg aatcagaaat ccttctatga tgcaagctat
121 ggctcacttc atgagacttg cacagatcag tttgtatctc tgagaacctc tgaaacgtca
181 aagatgtcca acttcacctt caaggagagc cgggtgacag tatcagcaac gtcaagcaac
241 gggaagattc tgaagaagag acggctgagt ttcagtgaga ccttcactga agatgacctg
301 cagtccataa cccatgatct ggaagagacc atccaaccca gatcagcacc ttacacctac
361 cagagtgatt tgagatacaa actgatgaag ctcgtcaggc agaagtttgt catgaatgat
421 tccctcaacc aaactatata tcaggatgtg gacaaacact atctcagcac cacttggtta
481 aatgacctgc aacaggaagt aaaatttgac atgtatgcct actcgtcggg aggagacgac
541 tctaaatatc ctgttactct aaaaatctca gattcacaac tgttcgtgag cgctcaagga
601 gaagaccagc ccgtgttgct gaaggagttg ccagaaacac caaaactcat cacaggtagt
661 gagaccgacc tcattttctt ctggaaaagt atcaactcta agaactactt cacatcagct
721 gcttatccag agctgtttat tgccaccaaa gaacaaagtc gggtgcacct ggcacgggga
781 ctgccctcta tgacagactt ccagatatca taa |
