| Primer Pair Descriptions: |
| PrimerBank ID |
6679695a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
128 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
CCTCCCGCCTTCTACAGGT |
19 |
62.7 |
448-466 |
| Reverse Primer |
CACACGGCACAGTAGCGAG |
19 |
63.0 |
575-557 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atgaccatga cccttcacac caaagcctcg ggaatggcct tgctgcacca gatccaaggg
61 aacgagctgg agcccctcaa ccgcccgcag ctcaagatgc ccatggagag ggccctgggc
121 gaggtatacg tggacaacag caagcccact gtgttcaact accccgaggg cgccgcctac
181 gagttcaacg ccgccgccgc cgccgccgcc gccgcctcgg cgccggtcta cggccagtcg
241 ggcatcgcct acggccccgg gtcggaggcg gccgccttca gtgccaacag cctgggggct
301 ttcccccagc tcaacagcgt gtcgcctagc ccgctgatgc tgctgcaccc gccgccgcag
361 ctgtctcctt tcctgcaccc gcacggccag caggtgccct actacctgga gaacgagccc
421 agcgcctacg ccgtgcgcga caccggccct cccgccttct acaggtctaa ttctgacaat
481 cgacgccaga atggccgaga gagactgtcc agcagtaacg agaaaggaaa catgatcatg
541 gagtctgcca aggagactcg ctactgtgcc gtgtgcaatg actatgcctc tggctaccat
601 tatggggtct ggtcctgcga aggctgcaag gctttcttta agagaagcat tcaaggacac
661 aatgactaca tgtgtccagc tacaaaccaa tgcaccattg acaagaaccg gaggaagagt
721 tgccaggcct gtcggctgcg caagtgttac gaagtgggca tgatgaaagg cggcatacgg
781 aaagaccgcc gaggagggag aatgttgaag cacaagcgtc agagagatga cttggaaggc
841 cgaaatgaaa tgggtgcttc aggagacatg agggctgcca acctttggcc aagccctctt
901 gtgattaagc acactaagaa gaatagccct gccttgtcct tgacagctga ccagatggtc
961 agtgccttgt tggatgctga accgcccatg atctattctg aatatgatcc ttctagaccc
1021 ttcagtgaag cctcaatgat gggcttattg accaacctag cagataggga gctggttcat
1081 atgatcaact gggcaaagag agtgccaggc tttggggact tgaatctcca tgatcaggtc
1141 caccttctcg agtgtgcctg gctggagatt ctgatgattg gtctcgtctg gcgctccatg
1201 gaacacccgg ggaagctcct gtttgctcct aacttgctcc tggacaggaa tcaaggtaaa
1261 tgtgtggaag gcatggtgga gatctttgac atgttgctgg ctacgtcaag tcggttccgc
1321 atgatgaacc tgcagggaga agagtttgtg tgcctcaaat ccatcatttt gcttaattcc
1381 ggagtgtaca cgtttctgtc cagcaccttg aagtctctgg aagagaagga ccacatccac
1441 cgtgtcctgg acaagatcac agacactttg atccacctga tggccaaagc tggcctgact
1501 ctgcagcagc agcatcgccg cctagctcag ctccttctca ttctttccca tatccggcac
1561 atgagtaaca aaggcatgga gcatctctac aacatgaaat gcaagaacgt tgtgcccctc
1621 tatgacctgc tcctggagat gttggatgcc caccgccttc atgccccagc cagtcgcatg
1681 ggagtgcccc cagaggagcc cagccagacc cagctggcca ccaccagctc cacttcagca
1741 cattccttac aaacctacta catacccccg gaagcagagg gcttccccaa cacgatctga |
