PrimerBank Search Result

The following primer pair is found for 6678355a1

Gene Descriptions:
GenBank Accession	NM_001163574
NCBI Protein Accession	NP_001157046
Species	Mouse
Coding DNA Length	5058
Gene Description	Mus musculus tight junction protein 1 (Tjp1), transcript variant 2, mRNA.

Primer Pair Descriptions:
PrimerBank ID	6678355a1
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data)
Amplicon Size	134
	Sequence (5' -> 3')	Length	Tm	Location
Forward Primer	GCCGCTAAGAGCACAGCAA	19	63.0	19-37
Reverse Primer	TCCCCACTCTGAAAATGAGGA	21	60.2	152-132
Location in Coding Sequence (primers and amplicon highlighted)
1 atgtccgcca gggccgcggc cgctaagagc acagcaatgg aggaaacagc tatatgggaa 61 cagcacacag tgacgcttca cagggctcct gggtttggat ttggaattgc aatctctggt 121 ggaagagata atcctcattt tcagagtggg gaaacctcca tagtgatttc tgatgtgtta 181 aaaggagggc cagctgaagg acagctacag gaaaatgacc gagttgcaat ggttaacgga 241 gtttcaatgg ataacgttga acatgctttt gctgttcagc agctaaggaa gagtgggaaa 301 aacgcaaaaa ttactatccg aaggaagaag aaagttcaga tccctgtaag tcacccagat 361 cctgagccgg tgtctgataa tgaagacgat agttatgacg aagaagtgca tgacccaaga 421 gctggccgcg gtgctttagc gaacagaagg agcgagaaga gctgggcaag ggataggagt 481 gcaagcaggg agaggagcct gtcccctcgc tcggacaggc ggtccgtggc ctccagtcag 541 cccgcaaagc ccaccaaggt cacactggtg aagtctcgga aaaatgaaga atatggtctt 601 cgattggcca gccatatatt tgtaaaggaa atttcacaag atagtttggc agcaagagat 661 ggtaacattc aagaagggga tgttgtcttg aagataaatg gtactgtgac agaaaatatg 721 tcattgacag atgcaaaaac actgatagaa aggtctaaag gcaagttaaa aatggtagtg 781 caaagagatg agcgggctac cttactgaac gtccctgacc tttcggatag tatccattct 841 gctaatgcct cggaaagaga tgacatttca gaaattcagt cactagcatc agaccattca 901 ggtcgctcgc atgacaggcc accccgccgc agccagtcac gatctcctga ccaacgttca 961 gagccctccg atcattccac gcagtctcca cagcagccca gcaatggcag tctccggagc 1021 agagaggaag agcgaatgtc taaacctggg gccatctcaa ctcctgtaaa acatgtagac 1081 gatcatccac ccaaagcagt ggaagaagtt acagttgaga aaaatgagaa gcagacgccc 1141 actcttccag aaccgaaacc tgtgtatgct caagttggac aaccagatgt ggatttaccc 1201 gtcagccctt ctgatggtgc tctgcctaat tcagctcatg aagacgggat acttaggccc 1261 agcatgaaac tggtaaaatt cagaaaagga gatagtgtgg gtttgcgact agctggtgga 1321 aatgatgtcg gaatatttgt agctggcgtt ctagaagata gccctgcagc caaagaaggc 1381 ttagaggaag gtgatcaaat tctcagggtg aacaatgtag atttcacaaa tatcataagg 1441 gaagaggccg tccttttcct ccttgacctc cctaaaggtg aagaagtgac catactggct 1501 cagaagaaga aggacgttta tcgccgcatt gtagaatcag atgtaggaga ttcattctat 1561 attagaacgc attttgaata tgaaaaagaa tctccttacg gacttagttt taacaaagga 1621 gaggtgttcc gggtcgtgga tactttatac aatggaaagc tgggctcttg gcttgccatt 1681 cgaattggca aaaatcataa ggaggtagaa cgaggcatca tccctaataa gaacagagct 1741 gaacagttag ccagtgtaca gtacacactt ccaaagacag cgggtggtga tcgggcagac 1801 ttctggaggt ttcgaggtct tcgcagctcc aagagaaatc ttcgaaaaag cagagaggac 1861 ttgtcagctc agccagttca aactaagttc ccagcttatg aaagggttgt tcttcgagaa 1921 gctggattcc taagacctgt aaccatcttt ggaccaatag ctgatgttgc cagagaaaag 1981 ttggcaagag aggagccaga tatctatcag attgcaaaaa gtgaaccacg agatgctggg 2041 actgaccatc gtagctctgg catcattcgc cttcatacaa taaagcaaat catagatcag 2101 gataaacatg ctttattaga tgtaacgcca aatgcggttg atcgtcttaa ttatgcgcag 2161 tggtatccaa ttgttgtgtt ccttaaccct gactctaagc aaggtgtaaa aacaatgagg 2221 atgaggttgt gtccggagtc tcggaaaagc gccaggaagc tatatgaacg ctctcataag 2281 cttcgtaaga acaatcacca tctcttcaca actacaatta acttaaactc aatgaatgat 2341 ggttggtacg gtgccctgaa agaagcgatt cagcagcaac agaaccagct ggtgtgggtc 2401 tctgagggga aggcggatgg tgctacaagt gatgaccttg atttgcatga cgatcgtctg 2461 tcctacctgt cagccccagg tagtgagtac tcaatgtata gcacggacag tagacacact 2521 tctgactatg aagacacaga tacagaaggc ggggcctaca ctgatcaaga actagatgaa 2581 actcttaatg atgaggtggg gactcccccg gagtctgcca ttacacggtc ctctgagcct 2641 gtaagagagg attcctctgg aatgcatcat gaaaaccaga cataccctcc ttactcacca 2701 caagcgcagc cacaagctat tcatagaata gactcccctg gacttaagcc agcctctcaa 2761 caggtgtaca ggaaggagcc gtattctgaa gaaatgatga gacaaaacca tattttaaaa 2821 caaccagctc ttggtcaccc agggcagagg ccagataaag agccaaatct agcctatgaa 2881 ccccaacttc catatataga aaaacaagcc agcagagacc ttgagcagcc gtcatacagg 2941 tatgaggtct caagctacac agaccagttt tctcggaact atgaccatcg cctacggttt 3001 gaagatcgaa tccctaccta tgaagaccag tggtcatatt atgatgacaa acagccctac 3061 caacctcggc cttttgagaa tcagcatccc cgagacctgg actccagaca acatcccgaa 3121 gaggcttcag aacgaggtta tttccagcgt tttgaagagc cagcccctct gtcgtacgac 3181 agtagaacac gctatgagca gctgcctcga acctctactc tacgacatga agagcagcca 3241 gcccctgcat atgaggtgca caacaggtac aggccagagg cacagcccta ttcttcaaca 3301 ggccctaagt catctgagcc caagcagtac tttgaccagt acccgcgaag ttatgagcaa 3361 gttccaccac caggctttac ctccaaaaca ggccattacg agcctctcca tggtgctgca 3421 gttgtccctc ctctgatacc ttcctctcaa caaaagccag aagtcctgcc ctcggctacc 3481 aaaccacagc ctccaccccc aaccctaact gaggaggagg aggatccagc aatgaaacca 3541 cagtctgtgc tcaccagagt caaaatgttt gaaaacaaaa gatctgcgtc tttggagaac 3601 aagaaagatg tgaatgacac tgccagcttt aagcctccgg aagtagcatc taaacctcca 3661 ggtgcttctc ttgctggccc taaacctgtc cctcagagtc agtttagtga gcacgacaaa 3721 acgctctaca ggctcccaga gcctcagaaa cctcaagtga agccacccga agatattgtt 3781 cgatcaaatc attacgaccc tgaagaggat gaagaatatt accggaaaca gctctcctac 3841 tttgaccgaa gaagttttga gagcaagcct tctgcacatc ttcctgctgg ccatcactca 3901 gagcctgcta agccagtcca ttctcagagc cagcccaact tctctagtta ctcttcaaag 3961 ggaaaacccg aaactgatgc tgtggataga tcattcagtg agaaacgtta tgatccagcc 4021 caggccacgc ctcctcctcc tccgttgccc tcacagtaca gccagccagc tccacctctg 4081 tccagctctt ctctccacat acattccaag ggcgcccagg gtgaaggcaa ctcagtatca 4141 ttggattttc agaactcata tatgtccaaa ccagacccac ccccatctca gagtaaacca 4201 gcaactttca gaccaccaac tcgagaagac ccccctcaga ctttctatcc gcagaaaagt 4261 ttcccagaca aagctccagt taacggagct gagcagactc agaaaaccat cactccggtg 4321 tacaatcgat tcacaccaaa gccgtacaca agttctgccc ggccatttga acgcaaattt 4381 gaaagtccga agttcaacca taatcttctg ccaagtgaaa ctgtacataa acctgaattg 4441 tcttcaaaaa ctcccacttc cccaaaaact cttatgaaag ctcatagttc aacacagcct 4501 ccagagtttg acagtggagt tgaaactttc tctgttcaca cagataagcc taaatatcaa 4561 atgaataata tcagcaccat gcctaaagct gtccctgtga gtccttcagc tgtggaagaa 4621 gatgaagatg aggatggtca tactgtagtg gctacagctc gtggcatttt taacagcaat 4681 ggtggtgtgt tgagttccat agaaaccggt gttagtataa ttatcccaca aggagccatt 4741 cctgaaggaa ttgagcaaga aatctatttc aaagtctgca gagacaatag cattctccca 4801 cctttagata aagagaaagg tgaaactctg ctgagccccc tagtgatgtg tgggccccat 4861 ggcctcaagt tcctgaagcc cgtggagcta cgcttgccac actgtgcgtc catgactcct 4921 gacggttggt cttttgctct aaaatcatcc gactcctcgt cgggtgaccc taaaacctgg 4981 caaaacaagt gtcttcctgg agatccgaat taccttgttg gagccaactg tgtttctgtc 5041 ctgattgacc acttttaa