| Primer Pair Descriptions: |
| PrimerBank ID |
6677709a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
111 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
AGGCTTCTGGGCCTTATGTG |
20 |
61.6 |
44-63 |
| Reverse Primer |
TGCTTCTCTCGCCAGGAATAC |
21 |
61.6 |
154-134 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atggacgatc tgtttcccct catctttccc tcagagccag cccaggcttc tgggccttat
61 gtggagatca tcgaacagcc gaagcaacgg ggcatgcgat tccgctataa atgcgagggg
121 cgctcagcgg gcagtattcc tggcgagaga agcacagata ccaccaagac acaccccacc
181 atcaagatca atggctacac aggaccagga acagttcgaa tctccctggt caccaaggat
241 ccacctcacc ggcctcatcc acatgaactt gtggggaagg actgccggga tggctactat
301 gaggctgacc tctgcccaga ccgcagtatc catagcttcc agaacctggg gatccagtgt
361 gtgaagaagc gagacctgga gcaagccatt agccagcgaa tccagaccaa caataacccc
421 tttcacgttc ctatagagga gcagcgcggg gactatgact tgaatgcagt gcgcctctgc
481 ttccaggtga cagtgcggga cccagcaggc aggcccctcc tcctgacccc tgtcctctca
541 catccgattt ttgataaccg ggcccccaac actgccgagc tcaagatctg ccgagtaaac
601 cggaactctg ggagctgcct cggtggggat gagatcttct tgctgtgcga caaggtgcag
661 aaagaagaca ttgaggtgta tttcacggga ccaggctggg aggcacgagg ctccttttct
721 caagctgatg tgcatcggca agtggccatt gtgttccgga ctcctccgta cgccgacccc
781 agcctccagg ctcctgttcg agtctccatg cagctacggc ggccttctga tcgcgagctc
841 agtgagccca tggagttcca gtacttgcca gacacagatg atcgccaccg gattgaagag
901 aagcgcaaaa ggacctatga gaccttcaag agtatcatga agaagagtcc tttcaatgga
961 ccaactgaac cccggcctcc aacccggcgt attgctgtgc ctacccgaaa ctcaacttct
1021 gtccccaagc cagccccgca gccctacacc ttcccagcat ccctcagcac catcaacttt
1081 gatgagtttt cccccatgct gttaccatca gggcagatct caaaccaggc cctggccttg
1141 gcaccgtcct ctgccccagt ccttgcccag accatggtcc cttcctcagc catggtacct
1201 ctggctcagc ccccagctcc tgccccagtt ctaaccccgg gtcctcccca gtccctgtct
1261 gcacctgttc caaagagcac ccaggctggg gaaggcacgc tgtcggaagc cctgctgcac
1321 ctgcagtttg atgctgatga agacttgggg gccttgcttg gcaacagcac agacccagga
1381 gtgttcacag acctggcatc tgtggacaac tcagagtttc agcagctcct gaaccagggt
1441 gtgtccatgt ctcactccac agctgagccc atgctgatgg agtaccctga agctataact
1501 cgcctggtga cagggtccca gaggccccct gacccagctc ccacacccct ggggacctcg
1561 gggcttccca atggtctctc aggagatgaa gacttctcct ccattgcgga catggacttc
1621 tctgctcttt tgagtcagat cagctcctaa |
