| Primer Pair Descriptions: |
| PrimerBank ID |
33990640a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
232 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
CTTCCTGACAAACGAGTCTGG |
21 |
60.3 |
4587-4607 |
| Reverse Primer |
CTGCCGAAACATCTCTGGGA |
20 |
61.6 |
4818-4799 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atggatgaac catctccgtt ggccaaaact ctggagctaa accagcactc ccgattcata
61 attgggtctg tgtctgaaga caactcagaa gatgagatca gtaacctggt gaagctggac
121 ctagaagaga aggagggctc cctgtcacca gcctccgtca gctcagatac actttctgat
181 ttggggatct ctggcttaca ggatggtttg gcctttcaca tgagatccag catgtctggc
241 ttgcacctag taaaacaagg tcgagacaga aagaaaatag actcacaacg agatttcact
301 gtggcttctc cagcagaatt tgttactcgt tttgggggaa ataaagtaat tgagaaggtt
361 cttatcgcca acaatggtat tgcagcagtg aaatgcatgc gatctatccg tcggtggtct
421 tatgaaatgt ttcgaaatga acgtgcaatc cgatttgttg tcatggttac acctgaagac
481 cttaaagcca atgcagaata cattaagatg gcagaccact atgttccagt gcctggagga
541 cccaacaaca acaattacgc aaatgtggag ttgattcttg atattgctaa aaggatacct
601 gtacaagcag tgtgggctgg ctggggtcat gcctctgaga acccgaaact cccagaactg
661 ctcttaaaaa atggcattgc tttcatgggc cctccaagcc aggccatgtg ggctttgggg
721 gataagattg catcttctat tgtggctcaa actgcaggta tcccaactct tccctggagt
781 ggcagtggtc ttcgagtgga ttggcaagaa aatgattttt cgaaacgtat cttaaatgtt
841 ccacaggatc tgtatgagaa aggctatgtg aaggatgtgg atgatggtct gaaggcagct
901 gaggaagttg gctatccagt gatgatcaag gcctcagagg gaggaggagg gaaagggatc
961 agaaaagtta acaatgcaga tgacttccct aacctcttca gacaggttca agctgaagtt
1021 cctggatcac ctatatttgt aatgagacta gcaaaacaat ctcgacatct ggaggtccag
1081 attctggcag atcagtatgg caatgctatt tctttgtttg gtcgtgactg ctctgtgcag
1141 cgcaggcatc agaagatcat tgaagaagct cctgctgcga ttgctacccc agcagtattt
1201 gaacacatgg aacagtgtgc agtgaaactt gccaaaatgg ttggttatgt gagtgctggg
1261 actgtggaat acttgtacag ccaggatgga agcttctact ttttggaact gaaccctcgg
1321 ctacaggttg aacatccttg tacagagatg gtggctgatg tcaatcttcc tgcagcacag
1381 ctccagattg ccatggggat ccctctattt aggatcaagg atattcgtat gatgtatggg
1441 gtatctcctt ggggagatgc tcccattgat tttgaaaatt ctgctcatgt tccttgccca
1501 aggggccacg tgattgctgc tcggatcacc agtgaaaacc cagatgaggg gtttaagccc
1561 agctctggaa cagttcagga acttaatttt cgtagcaata agaacgtttg gggttatttc
1621 agtgttgctg ctgctggagg acttcatgaa tttgctgatt ctcagttcgg gcactgcttt
1681 tcctggggag aaaacaggga ggaagcaatc tcaaatatgg tggtggcact gaaggagctg
1741 tctattcggg gtgactttcg aactacagtg gaatacctca tcaaactgct ggagacagaa
1801 agctttcagc ttaacagaat cgacactggc tggctggaca gactgatcgc agagaaagtg
1861 caggcagagc gacctgacac catgttggga gttgtgtgtg gggctctcca tgtagcagat
1921 gtgagcctga ggaacagcat ctctaacttc cttcactcct tagagagggg tcaagtcctt
1981 cctgctcaca cacttctgaa cacagtagat gttgaactta tctatgaagg aatcaaatat
2041 gtacttaagg tgactcggca gtctcccaac tcctacgtag tgataatgaa tggctcgtgt
2101 gtggaagtgg atgtgcatcg gctgagtgat ggtggcctgc tcttgtctta tgacggcagc
2161 agttacacca catacatgaa ggaagaggtg gacagatatc gaatcacaat tggcaataaa
2221 acctgtgtgt ttgagaagga aaatgaccca tctgtaatgc gctcaccgtc tgctgggaag
2281 ttaatccagt atattgtgga agatggcgga catgtgtttg ctggccagtg ctatgctgag
2341 attgaggtaa tgaagatggt gatgacttta acagctgtag aatctggctg catccattat
2401 gtcaaacgac ctggagcagc acttgaccct ggctgtgtga tagccaaaat gcaactggac
2461 aaccccagta aagttcaaca ggctgagctt cacacgggca gtctaccaca gatccagagc
2521 acagctctca gaggcgagaa gctccatcga gttttccact atgtcctgga taacctggtc
2581 aatgtgatga atggatactg ccttccagac cctttcttca gcagcagggt aaaagactgg
2641 gtagaaagat tgatgaagac tctgagagac ccctccttgc ctctgctaga gctgcaggat
2701 atcatgacca gtgtctctgg ccggatcccc ctcaatgtgg agaagtctat taagaaggaa
2761 atggctcagt atgctagcaa catcacatca gtcctgtgtc agtttcccag ccagcagatt
2821 gccaacatcc tagatagtca tgcagctaca ctgaaccgga aatctgagcg ggaagtcttc
2881 ttcatgaaca cccagagcat tgtccagctg gtgcagaggt accgaagtgg catccgtggc
2941 cacatgaagg ctgtggtgat ggatctgctg cggcagtacc tgcgagtaga gacacagttt
3001 cagaacggcc actacgacaa atgtgtattc gcccttcggg aagagaacaa aagcgacatg
3061 aacaccgtac tgaactacat cttctcccac gctcaggtca ccaaaaagaa tctcctggtg
3121 acaatgctta ttgatcagtt atgtggccgg gaccctacac ttactgatga gctgctaaat
3181 atcctcacag agctaaccca actcagcaag accaccaacg ctaaagtggc gctgcgcgct
3241 cgtcaggttc ttattgcttc ccatttgcca tcatatgagc ttcgccataa ccaagtagag
3301 tctatcttcc tatcagccat tgacatgtat ggacaccagt tttgcattga gaacctgcag
3361 aaactcatcc tctcggaaac atctattttc gatgtcctcc caaacttttt ttaccacagc
3421 aaccaggtgg tgaggatggc agctctggag gtgtatgttc gaagggctta cattgcctat
3481 gaactcaaca gcgtacaaca ccgccagctt aaggacaaca cctgtgtggt ggaatttcag
3541 ttcatgctgc ccacatccca tccaaacaga gggaacatcc ccacgctaaa cagaatgtcc
3601 tttgcctcca acctcaacca ctatggcatg actcatgtag ctagtgtcag cgatgttctg
3661 ttggacaacg ccttcacgcc accttgtcag cggatgggcg gaatggtctc tttccggacc
3721 tttgaagatt ttgtcaggat ctttgatgaa ataatgggct gcttctgtga ctccccaccc
3781 caaagcccca cattcccaga gtctggtcat acttcgctct atgatgaaga caaggtcccc
3841 agggatgaac caatacatat tctgaatgtg gctatcaaga ctgatggcga tattgaggat
3901 gacaggcttg cagctatgtt cagagagttc acccagcaga ataaagctac tttggttgag
3961 catggcatcc ggcgacttac gttcctagtt gcacaaaagg atttcagaaa acaagtcaac
4021 tgtgaggtgg atcagagatt tcatagagaa ttccccaaat ttttcacatt ccgagcaagg
4081 gataagtttg aggaggaccg catttatcga cacctggagc ctgctctggc tttccagtta
4141 gagttgaacc ggatgagaaa ttttgacctt actgccatcc catgtgctaa tcacaagatg
4201 cacctgtacc ttggggctgc taaggtggaa gtaggcacag aagtgactga ctacaggttc
4261 tttgttcgtg cgatcatcag gcactctgat ctggtcacaa aggaagcttc tttcgaatat
4321 ctacaaaatg aaggggaacg actgctcctg gaagctatgg atgaattgga agttgctttt
4381 aataatacaa atgtccgcac tgactgtaac cacatcttcc tcaactttgt gcccacggtc
4441 atcatggacc catcaaagat tgaagaatct gtgcggagca tggtaatgcg ctatggaagt
4501 cggctatgga aattgcgggt cctccaggca gaactgaaaa tcaacattcg cctgacaaca
4561 actggaaaag caattcccat ccgcctcttc ctgacaaacg agtctggcta ctacttggac
4621 atcagcctgt ataaggaagt gactgactcc aggacagcac agatcatgtt tcaggcatat
4681 ggagacaagc agggaccact gcatggaatg ttaattaata ctccatatgt gaccaaagac
4741 cttcttcaat caaagaggtt ccaggcacag tccttaggaa caacatatat atatgatatc
4801 ccagagatgt ttcggcagtc actcatcaaa ctctgggagt ccatgtccac ccaagcattt
4861 cttccttcgc ctcctttgcc ttccgacatc ctgacgtata ctgaactggt gttggatgat
4921 caaggccagc tggtccatat gaacagactt ccaggaggaa atgagattgg catggtagcc
4981 tggaaaatga gccttaaaag ccctgaatat ccagatggcc gagatatcat tgtcatcggc
5041 aatgacatta catatcggat cggttccttt gggcctcagg aggatttgct gtttctcaga
5101 gcttctgaac ttgccagagc agaaggcatc ccacgcatct acgtggcagc gaacagtgga
5161 gctagaattg gacttgcaga agaaatacgc catatgttcc atgtggcctg ggtagatcct
5221 gaagatccct acaagggata caagtattta tatctgacac cccaggatta taagagagtc
5281 agtgccctca attctgtcca ctgtgaacat gtggaggatg aaggggaatc caggtacaag
5341 ataacagata ttatcgggaa agaagaagga cttggagcag agaaccttcg gggttctgga
5401 atgattgctg gggaatcctc attggcttac gatgaggtca tcaccatcag cctggttaca
5461 tgccgggcca ttggtattgg ggcttacctt gtccggctgg gacaaagaac catccaggtt
5521 gagaattctc acttaattct gacaggagca ggtgccctca acaaagtcct tggtcgggaa
5581 gtatacacct ccaacaacca gcttgggggc atccagatta tgcacaacaa tggggttacc
5641 cactccactg tttgtgatga ctttgaggga gtcttcacag tcttacactg gctgtcatac
5701 atgccgaaga gcgtacacag ttcagttcct ctcctgaatt ccaaggatcc tatagataga
5761 atcatcgagt ttgttcccac aaaggcccca tatgatcctc ggtggatgct agcaggccgt
5821 cctcacccaa cccagaaagg ccaatggttg agtgggtttt ttgactatgg atctttctca
5881 gaaatcatgc agccctgggc acagaccgtg gtagttggca gagccaggtt agggggaata
5941 cccgtgggag tagttgctgt agaaacccga acagtggaac tcagtatccc agctgatcct
6001 gcgaacctgg attctgaagc caagataatc cagcaggccg gccaggtttg gttcccagac
6061 tctgcattta agacctatca agctatcaag gactttaacc gtgaagggct acctctaatg
6121 gtctttgcca actggagagg tttctctggt gggatgaaag atatgtatga ccaagtgctc
6181 aagtttggcg cttacattgt ggatggcttg cgggaatgtt cccagcctgt aatggtttac
6241 atcccgcccc aggctgagct tcggggtggt tcttgggttg tgatcgaccc caccatcaac
6301 cctcggcaca tggagatgta cgctgaccga gaaagcaggg gatctgttct ggagccagaa
6361 gggacagtag aaatcaaatt ccgtaaaaag gatctggtga aaaccatgcg tcgggtagat
6421 ccagtttaca tccgcttggc tgagcgattg ggcaccccag agctaagccc cactgagcgg
6481 aaggagctgg agagcaagtt gaaggagcgg gaggagttcc taattcccat ttaccatcag
6541 gtagctgtgc agtttgctga cttgcacgac acaccaggcc ggatgcagga gaagggtgtc
6601 attaacgata tcttggattg gaaaacatcc cgcaccttct tctactggag gctgaggcgc
6661 ctcctgctgg aggacctggt caagaagaaa atccacaatg ccaaccctga gctgactgac
6721 ggccagatcc aggccatgtt gagacgctgg tttgtagaag ttgaaggcac agtgaaggct
6781 tacgtctggg acaataataa ggacctggtg gagtggctgg agaagcaact gacagaggaa
6841 gatggcgtcc gctctgtgat agaggagaac atcaaataca tcagcaggga ctatgtcctg
6901 aagcagatcc gcagcttggt ccaggccaac ccagaagtcg ccatggactc catcgtccac
6961 atgacccagc acatctcacc cacccagcga gcagaagttg taaggatcct ctccactatg
7021 gattcccctt ccacgtag |
