| Primer Pair Descriptions: |
| PrimerBank ID |
27229121a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
135 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
TCATCTCCTACGTGGTCGC |
19 |
60.8 |
71-89 |
| Reverse Primer |
CTGCGCCAAGAAATGCAGAG |
20 |
62.0 |
205-186 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atgctgtgct tcctcagggg aatggctttc gtccccttcc tcctggtgac ttggtcgtcc
61 gcagccttca tcatctccta cgtggtcgcg gtgctctctg ggcacgtcaa cccctttctc
121 ccctatatca gtgacacagg aacaactcct ccagagagtg gtatttttgg attcatgata
181 aacttctctg catttcttgg cgcagctacg atgtacacaa gatacaagat agtggagaag
241 cagaatgaga cctgctactt cagcactccc gtttttaact tggtgtcctt ggcgcttgga
301 ttggtgggat gcatcggaat gggcatcgta gccaacttcc aggagttagc cgtgcctgtg
361 gtccatgatg gcggtgcgct tctggctttc gtctgcgggg tggtgtacac gctcctgcaa
421 tcgatcatct cctacaaatc ctgtccccag tggaacagtc tcaccacgtg ccatgtcagg
481 atggccatct ccgctgtttc gtgcgcagct gtcgtcccca tgattgcctg tgcttcactc
541 atttctataa ccaagctgga atggaatcca aaagaaaagg attatatata tcacgtggtg
601 agcgccatct gtgagtggac cgtggctttt ggttttattt tctatttcct aacattcatc
661 caagatttcc agagtgtcac tctaaggata tccacagaaa tcaatgacga cttttga |
