| Primer Pair Descriptions: |
| PrimerBank ID |
26342390a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
244 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
CGATGGCCTACAGGGGATAAC |
21 |
61.7 |
325-345 |
| Reverse Primer |
ACAGTAGAGAGGCTAGAGCTAGA |
23 |
60.6 |
568-546 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atgagcgttg catgtgtctt gaagagaaaa gcagtgcttt ggcaggactc tttcagcccc
61 cacctgaaac atcaccctca agaaccagct aatcccaaca tgcctgttgt tttgacatct
121 ggaacagggt cgcaagcgca gccacagcca gctgcaaatc aggctcttgc agctggaaca
181 cactccagcc ctgtcccagg atccatagga gttgcaggcc gttcccagga cgacgctatg
241 gtggactact tctttcagag gcagcatggt gagcagcttg ggggaggagg aagtggtgga
301 ggcggctata atactagcaa acatcgatgg cctacagggg ataacattca tgcagaacac
361 caggtacgct ctatggatga attgaatcat gattttcaag cacttgctct ggaagggaga
421 gctatgggcg agcagctctt gccaggtaaa aagttttggg aaacagatga atccagcaaa
481 gatggaccaa aaggaatatt cctgggtgat caatggcgag acagtgcctg gggaacatca
541 ggtaatctag ctctagcctc tctactgtga |
