Primer Pair Descriptions: |
PrimerBank ID |
26337575a1 |
Validation Results  (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
Amplicon Size |
138 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
Forward Primer |
ATGTCACTTCTGACCTCAGGAT |
22 |
60.2 |
1-22 |
Reverse Primer |
GTGAACGAGTCCCACACCA |
19 |
61.5 |
138-120 |
Location in Coding Sequence (primers and amplicon highlighted) |
1 atgtcacttc tgacctcagg atatgtcaac attttaggga ctgagcattt taaggggtgt
61 tttagaatgc tgttcttccc tgatctctcc tttatgcttt taagctgtat ttcctgttct
121 ggtgtgggac tcgttcacag aatcagagca cggagggact ctgtcgtggt gcacgttggt
181 tgtctgatgt gtgataaatc catggtttta tggctaactt tacattttcc agaggaaagg
241 aaacggattt ctagtgtcca gaccaaaaga gccattcacc cagagttctc acctgttgta
301 tactggtctg atgaaacctg ccctgactcg gcctaa |