PrimerBank Search Result

The following primer pair is found for 23956044a1

Gene Descriptions:
GenBank Accession	NM_009778
NCBI Protein Accession	NP_033908
Species	Mouse
Coding DNA Length	4992
Gene Description	Mus musculus complement component 3 (C3), mRNA.

Primer Pair Descriptions:
PrimerBank ID	23956044a1
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data)
Amplicon Size	159
	Sequence (5' -> 3')	Length	Tm	Location
Forward Primer	CCAGCTCCCCATTAGCTCTG	20	61.8	50-69
Reverse Primer	GCACTTGCCTCTTTAGGAAGTC	22	60.9	208-187
Location in Coding Sequence (primers and amplicon highlighted)
1 atgggaccag cttcagggtc ccagctacta gtgctactgc tgctgttggc cagctcccca 61 ttagctctgg ggatccccat gtattccatc attactccca atgtcctacg gctggagagc 121 gaagagacca tcgtactgga ggcccacgat gctcagggtg acatcccagt cacagtcact 181 gtgcaagact tcctaaagag gcaagtgctg accagtgaga agacagtgtt gacaggagcc 241 agtggacatc tgagaagcgt ctccatcaag attccagcca gtaaggaatt caactcagat 301 aaggaggggc acaagtacgt gacagtggtg gcaaacttcg gggaaacggt ggtggagaaa 361 gcagtgatgg taagcttcca gagtgggtac ctcttcatcc agacagacaa gaccatctac 421 acccctggct ccactgtctt atatcggatc ttcactgtgg acaacaacct actgcccgtg 481 ggcaagacag tcgtcatcct cattgagacc cccgatggca ttcctgtcaa gagagacatt 541 ctgtcttcca acaaccaaca cggcatcttg cctttgtctt ggaacattcc tgaactggtc 601 aacatggggc agtggaagat ccgagccttt tacgaacatg cgccgaagca gatcttctcc 661 gcagagtttg aggtgaagga atacgtgctg cccagttttg aggtccgggt ggagcccaca 721 gagacatttt attacatcga tgacccaaat ggcctggaag tttccatcat agccaagttc 781 ctgtacggga aaaacgtgga cgggacagcc ttcgtgattt ttggggtcca ggatggcgat 841 aagaagattt ctctggccca ctccctcacg cgcgtagtga ttgaggatgg tgtgggggat 901 gcagtgctga cccggaaggt gctgatggag ggggtacggc cttccaacgc cgacgccctg 961 gtggggaagt ccctgtatgt ctccgtcact gtcatcctgc actcaggtag tgacatggta 1021 gaggcagagc gcagtgggat cccgattgtc acttccccgt accagatcca cttcaccaag 1081 acacccaaat tcttcaagcc agccatgccc tttgacctca tggtgttcgt gaccaacccc 1141 gatggctctc cggccagcaa agtgctggtg gtcactcagg gatctaatgc aaaggctctc 1201 acccaagatg atggcgtggc caagctaagc atcaacacac ccaacagccg ccaacccctg 1261 accatcacag tccgcaccaa gaaggacact ctcccagaat cacggcaggc caccaagaca 1321 atggaggccc atccctacag cactatgcac aactccaaca actacctaca cttgtcagtg 1381 tcacgaatgg agctcaagcc gggggacaac ctcaatgtca acttccacct gcgcacagac 1441 ccaggccatg aggccaagat ccgatactac acctacctgg ttatgaacaa ggggaagctc 1501 ctgaaggcag gccgccaggt tcgggagcct ggccaggacc tggtggtctt gtccctgccc 1561 atcactccag agtttattcc ttcatttcgc ctggtggctt actacaccct gattggagct 1621 agtggccaga gggaggtggt ggctgactct gtgtgggtgg atgtgaagga ttcctgtatt 1681 ggcacgctgg tggtgaaggg tgacccaaga gataaccatc tcgcacctgg gcaacaaacg 1741 acactcagga ttgaaggaaa ccagggggcc cgagtggggc tagtggctgt ggacaaggga 1801 gtgtttgtgc tgaacaagaa gaacaaactc acacagagca agatctggga tgtggtagag 1861 aaggcagaca ttggctgcac cccaggcagt gggaagaact atgctggtgt cttcatggat 1921 gcaggcctgg ccttcaagac aagccaagga ctgcagactg aacagagagc agatcttgag 1981 tgcaccaagc cagcagcccg ccgccgtcgc tcagtacagt tgatggaaag aaggatggac 2041 aaagctggtc agtacactga caagggtctt cggaagtgtt gtgaggatgg tatgcgggat 2101 atccctatga gatacagctg ccagcgccgg gcacgcctca tcacccaggg cgagaactgc 2161 ataaaggcct tcatagactg ctgcaaccac atcaccaagc tgcgtgaaca acacagaaga 2221 gaccacgtgc tgggcctggc caggagtgaa ttggaggaag acataattcc agaagaagat 2281 attatctcta gaagccactt cccacagagc tggttgtgga ccatagaaga gttgaaagaa 2341 ccagagaaaa atggaatctc tacgaaggtc atgaacatct ttctcaaaga ttccatcacc 2401 acctgggaga ttctggcagt gagcttgtca gacaagaaag ggatctgtgt ggcagacccc 2461 tatgagatca gagtgatgca ggacttcttc attgacctgc ggctgcccta ctctgtagtg 2521 cgcaacgaac aggtggagat cagagctgtg ctcttcaact accgtgaaca ggaggaactt 2581 aaggtgaggg tggaactgtt gcataatcca gccttctgca gcatggccac cgccaagaat 2641 cgctacttcc agaccatcaa aatccctccc aagtcctcgg tggctgtacc gtatgtcatt 2701 gtccccttga agatcggcca acaagaggtg gaggtcaagg ctgctgtctt caatcacttc 2761 atcagtgatg gtgtcaagaa gacactgaag gtcgtgccag aaggaatgag aatcaacaaa 2821 actgtggcca tccatacact ggacccagag aagctcggtc aagggggagt gcagaaggtg 2881 gatgtgcctg ccgcagacct tagcgaccaa gtgccagaca cagactctga gaccagaatt 2941 atcctgcaag ggagcccggt ggttcagatg gctgaagatg ctgtggacgg ggagcggctg 3001 aaacacctga tcgtgacccc cgcaggctgt ggggaacaga acatgattgg catgacacca 3061 acagtcattg cggtacacta cctggaccag accgaacagt gggagaagtt cggcatagag 3121 aagaggcaag aggccctgga gctcatcaag aaagggtaca cccagcagct ggccttcaaa 3181 cagcccagct ctgcctatgc tgccttcaac aaccggcccc ccagcacctg gctgacagcc 3241 tacgtggtca aggtcttctc tctagctgcc aacctcatcg ccatcgactc tcacgtcctg 3301 tgtggggctg ttaaatggtt gattctggag aaacagaagc cggatggtgt ctttcaggag 3361 gatgggcccg tgattcacca agaaatgatt ggtggcttcc ggaacgccaa ggaggcagat 3421 gtgtcactca cagccttcgt cctcatcgca ctgcaggaag ccagggacat ctgtgagggg 3481 caggtcaata gccttcctgg gagcatcaac aaggcagggg agtatattga agccagttac 3541 atgaacctgc agagaccata cacagtggcc attgctgggt atgccctggc cctgatgaac 3601 aaactggagg aaccttacct cggcaagttt ctgaacacag ccaaagatcg gaaccgctgg 3661 gaggagcctg accagcagct ctacaacgta gaggccacat cctacgccct cctggccctg 3721 ctgctgctga aagactttga ctctgtgccc cctgtagtgc gctggctcaa tgagcaaaga 3781 tactacggag gcggctatgg ctccacccag gctaccttca tggtattcca agccttggcc 3841 caatatcaaa cagatgtccc tgaccataag gacttgaaca tggatgtgtc cttccacctc 3901 cccagccgta gctctgcaac cacgtttcgc ctgctctggg aaaatggcaa cctcctgcga 3961 tcggaagaga ccaagcaaaa tgaggccttc tctctaacag ccaaaggaaa aggccgaggc 4021 acattgtcgg tggtggcagt gtatcatgcc aaactcaaaa gcaaagtcac ctgcaagaag 4081 tttgacctca gggtcagcat aagaccagcc cctgagacag ccaagaagcc cgaggaagcc 4141 aagaatacca tgttccttga aatctgcacc aagtacttgg gagatgtgga cgccactatg 4201 tccatcctgg acatctccat gatgactggc tttgctccag acacaaagga cctggaactg 4261 ctggcctctg gagtagatag atacatctcc aagtacgaga tgaacaaagc cttctccaac 4321 aagaacaccc tcatcatcta cctagaaaag atttcacaca ccgaagaaga ctgcctgacc 4381 ttcaaagttc accagtactt taatgtggga cttatccagc ccgggtcggt caaggtctac 4441 tcctattaca acctcgagga atcatgcacc cggttctatc atccagagaa ggacgatggg 4501 atgctcagca agctgtgcca cagtgaaatg tgccggtgtg ctgaagagaa ctgcttcatg 4561 caacagtcac aggagaagat caacctgaat gtccggctag acaaggcttg tgagcccgga 4621 gtcgactatg tgtacaagac cgagctaacc aacatagagc tgttggatga ttttgatgag 4681 tacaccatga ccatccagca ggtcatcaag tcaggctcag atgaggtgca ggcagggcag 4741 caacgcaagt tcatcagcca catcaagtgc agaaacgccc tgaagctgca gaaagggaag 4801 aagtacctca tgtggggcct ctcctctgac ctctggggag aaaagcccaa caccagctac 4861 atcattggga aggacacgtg ggtggagcac tggcctgagg cagaagaatg ccaggatcag 4921 aagtaccaga aacagtgcga agaacttggg gcattcacag aatctatggt ggtttatggt 4981 tgtcccaact ga