| Primer Pair Descriptions: |
| PrimerBank ID |
22902405a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
337 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
AGACTTCGAGCAGTTAGGATGC |
22 |
61.8 |
32-53 |
| Reverse Primer |
AAGCTGCCCCAAAGCTCAC |
19 |
62.9 |
368-350 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 ccacgcgtcc gcccacgcgt ccggatcggt gagacttcga gcagttagga tgccgcgtgg
61 aagccgaagc cgcacttccc gggtgactcc tccggccagc cgggcccctc agatgagggc
121 tgctccccga agagcacctg cagctcagcc tccagcagca gctgcgccat ctgcagttgg
181 ctcacctgcc gctgcgcccc ggcagccagg cctgatggcc cagatggcta ccaccgcggc
241 cggtgtggct gtgggctctg cagtgggaca caccctgggt cacgccatca ctgggggctt
301 cagcggaggt ggcagtgctg agcccgcaaa gcccgacatc acttaccagg tgagctttgg
361 ggcagcttct cctttctga |
