| Primer Pair Descriptions: |
| PrimerBank ID |
22094089a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
104 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
GGCCTTGGAAGCATGTAGAGG |
21 |
62.5 |
83-103 |
| Reverse Primer |
GGAGAACTCGTTAGAGACGACTT |
23 |
61.1 |
186-164 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atgtggctgc agaatttact tttcctgggc attgtggtct acagcctctc agcacccacc
61 cgctcaccca tcactgtcac ccggccttgg aagcatgtag aggccatcaa agaagccctg
121 aacctcctgg atgacatgcc tgtcacgttg aatgaagagg tagaagtcgt ctctaacgag
181 ttctccttca agaagctaac atgtgtgcag acccgcctga agatattcga gcagggtcta
241 cggggcaatt tcaccaaact caagggcgcc ttgaacatga cagccagcta ctaccagaca
301 tactgccccc caactccgga aacggactgt gaaacacaag ttaccaccta tgcggatttc
361 atagacagcc ttaaaacctt tctgactgat atcccctttg aatgcaaaaa accaggccaa
421 aaatga |
