| Primer Pair Descriptions: |
| PrimerBank ID |
21699058a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
159 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
GACGGGGACATGGGAGAGA |
19 |
62.4 |
16-34 |
| Reverse Primer |
GTAAAACAGAGCCAACGGGTAG |
22 |
60.9 |
174-153 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atggcgtcta cagcggacgg ggacatggga gagacgctgg agcagatgcg ggggctgtgg
61 ccgggtgtcg aggatctgag ccttaacaag ttggcgacgt ctctgggcgc gtcggaacag
121 gcgctgcggc tcatcttctc catcttcctg ggctacccgt tggctctgtt ttaccggcat
181 taccttttct acaaggacag ctacctcatc catctcttcc acaccttcac gggcctctca
241 attgcttatt tcaactttgg ccaccagttc taccactcct tgctatgtgt cgtgcttcag
301 ttcctcatcc tgcgactcat gggccgcacc gtcactgccg ttattactac cctttgcttc
361 cagatggcct accttcttgc cggatattac tacacagcca ccggtgacta cgatatcaag
421 tggacaatgc cacattgtgt cttgacactg aagctaattg ggctgtgtat tgactactac
481 gatggaggca aagacgggaa ttccttgacc tctgagcaac agaaatatgc catacggggt
541 gtcccttcat tgctggaagt tgctggcttc tcctacttct atggagcctt cttggtaggg
601 ccccaatttt caatgaacca ctacatgaag ctggtgcggg gacagctgac tgacatacca
661 gggaagatgc caaacagcac catacctgct ctcaagcgcc tgagtctggg ccttgtctac
721 ctggtgggct acaccctgct gagcccccac atcacagacg actatctcct cacagaagac
781 tatgataacc gccctttctg gttccgctgc atgtacatgc tgatctgggg caaatttgtg
841 ctgtacaaat acgtcacctg ctggctggtc acagaaggag tgtgcattct gtcgggcctg
901 ggctttaatg gcttcgatga aaatgggacc gtgagatggg atgcctgtgc caacatgaaa
961 gtgtggctct ttgaaaccac ccctcgcttc aatggcacca tcgcctcttt caacatcaat
1021 accaatgcct gggtagcccg ttacatcttc aaacgcctca agttccttgg aaataaagag
1081 ctctcacaag gtctctcctt gctgttcttg gccctctggc atggcctaca ctcaggatac
1141 ctgatttgct tccagatgga attcctcatt gttatcgtgg aaaagcaggt cagcagtcta
1201 attcgggaca gccctgccct gagcagcctg gcctccatca ctgccctaca gcccttctac
1261 tacttggtgc aacagaccat ccactggctg ttcatgggtt actctatgac tgccttctgc
1321 ctcttcacat gggacaaatg gcttaaggtg tacagatcca tctatttcct tggacatgtc
1381 ttcttcttga gcctactatt catattgcct tatatccaca aagcaatggt gccaagaaaa
1441 gaaaagttaa aaaagaggga atga |
