| Primer Pair Descriptions: |
| PrimerBank ID |
21450153a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
187 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
CTGGAGCAGGGAGGCTTATTT |
21 |
61.6 |
436-456 |
| Reverse Primer |
GGACAACAAAGTCACTGGTACAG |
23 |
61.0 |
622-600 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atgcggggtg cggtgtgggc ggcccggagg cgcgcggggc agcagtggcc tcggtccccg
61 ggccctgggc cgggtccgcc cccgccgcca ccgctgctgt tgctgctact actgctgctg
121 ggcggcgcga gcgctcagta ctccagcgac ctgtgcagct ggaaggggag tgggctcacc
181 cgagaggcac gcagcaagga ggtggagcag gtgtacctgc gctgctccgc aggctctgtg
241 gagtggatgt acccaactgg ggcgctcatt gttaacctac ggcccaacac cttctcacct
301 gcccagaact tgactgtgtg catcaagcct ttcagggact cctctggagc caatatttat
361 ttggaaaaaa ctggagaact aagactgttg gtgcgggaca tcagaggtga gcctggccaa
421 gtgcagtgct tcagcctgga gcagggaggc ttatttgtgg aggcgacacc ccaacaggac
481 atcagcagaa ggaccacagg cttccagtat gagctgatga gtgggcagag gggactggac
541 ctgcacgtgc tgtctgcccc ctgtcggcct tgcagtgaca ctgaggtcct ccttgccatc
601 tgtaccagtg actttgttgt ccgaggcttc attgaggacg tcacacatgt accagaacag
661 caagtgtcag tcatctacct gcgggtgaac aggcttcaca ggcagaagag cagggtcttc
721 cagccagctc ctgaggacag tggccactgg ctgggccatg tcacaacact gctgcagtgt
781 ggagtacgac cagggcatgg ggaattcctc ttcactggac atgtgcactt tggggaggca
841 caacttggat gtgccccacg ctttagtgac tttcaaagga tgtacaggaa agcagaagaa
901 atgggcataa acccctgtga aatcaatatg gagtga |
