| Primer Pair Descriptions: |
| PrimerBank ID |
21312216a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
170 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
CCATCGGCCTTTGAGAAAGTG |
21 |
61.2 |
4-24 |
| Reverse Primer |
ACACATGAATAACGGGGTTTCC |
22 |
60.6 |
173-152 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atgccatcgg cctttgagaa agtggtcaag aatgtgatca aggaggtaag cggcagcaga
61 ggcgatctca ttccggtgga cagcctgcgg aactccacca gcttcaggcc ctactgcctt
121 ctgaacagga aattttcaag ctcaaggttc tggaaacccc gttattcatg tgtcaacctg
181 tcaatcaagg acatcctgga gcccagtgct ccagaaccag aaccggagtg ttttggctcc
241 ttcaaagtct ctgatgtcgt cgatgggaac attcagggca gagtgatgtt gtcaggcatg
301 ggagaaggga aaatttctgg tggggctgca gtgtctgaca gttccagtgc ctccatgaat
361 gtgtgtatac tgcgtgtgac tcagaagacc tgggagacca tgcagcatga aaggcacctt
421 cagcagcctg agaacaaaat cctgcaacag cttcggagtc gtggggatga cctgtttgtg
481 gtgaccgagg tgctgcagac aaaggaggaa gtgcagatca ctgaggtcca cagccaagag
541 ggctcaggcc agtttacgct gcctggagct ttatgcttga agggtgaagg caagggccac
601 caaagccgga agaagatggt gaccattcct gcaggcagca tcctggcatt ccgagtggcc
661 caactgctta ttggctctaa atgggatatc cttctcgtct cagatgagaa acagaggacc
721 tttgagccct cctcaggtga cagaaaagca gtgggccaga ggcaccatgg cctcaatgtg
781 cttgctgcgc tttgttccat cggaaagcag ctcagtctcc tgtcagatgg gattgatgag
841 gaggaattaa ttgaggcggc agacttccag ggcctgtatg ctgaggtgaa ggcttgctcc
901 tcagaactgg agagcttgga aatggagttg agacaacaga tactggtgaa catcggaaag
961 attttacagg accagcccag catggaagcc ttagaggcct cactagggca gggcctgtgc
1021 agtggcggcc aggtggagcc tctggacggc ccagctggct gcatccttga gtgtctggtg
1081 cttgactctg gagaactggt gccggaactc gcagccccta tcttctacct gctgggagca
1141 ctggctgtgc tgagtgaaac ccagcagcag ctgctagcta aggctctgga gacaacggtg
1201 ctgtcaaagc agctggagtt ggtgaagcac gtcttggaac agagcacccc gtggcaggag
1261 cagagttctg tgtccctgcc caccgtgctc cttggggact gctgggatga aaagaatccc
1321 acctgggtct tgctagaaga atgtggccta aggctgcagg tagaatcccc ccaggtgcac
1381 tgggaaccaa cgtctctgat ccccacaagt gcgctctatg cctccctgtt cctattgtca
1441 agtctaggcc agaaaccttg ttag |
