| Primer Pair Descriptions: |
| PrimerBank ID |
13097057a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
61 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
TGACCCGGCTATTCCGTGA |
19 |
62.7 |
2369-2387 |
| Reverse Primer |
CTGGGCTGAGCAATACAGTTC |
21 |
60.7 |
2429-2409 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atggacgagc tggccttcgg tgaggcggct ctggaacaga cactggccga gatgtgcgaa
61 ctggacacag cggttttgaa cgacatcgaa gacatgctcc agctcatcaa caaccaagac
121 agtgacttcc cgggcctgtt tgacgccccc tatgctgggg gtgagacagg ggacacaggc
181 cccagcagcc caggtgccaa ctctcctgag agcttctctt ctgcttctct ggcctcctct
241 ctggaagcct tcctgggagg acccaaggtg acacctgcac ccttgtcccc tccaccatcg
301 gcacccgctg ctttaaagat gtacccgtcc gtgtccccct tttcccctgg gcctgggatc
361 aaagaggagc cagtgccact caccatccta cagcctgcag cgccacagcc gtcaccgggg
421 accctcctgc ctccgagctt ccccgcacca cccgtacagc tcagccctgc gcccgtgctg
481 ggttactcga gcctgccttc aggcttctca gggacccttc caggaaacac tcagcagcca
541 ccatctagcc tgccgctggc ccctgcacca ggagtcttgc ccacccctgc cctgcacacc
601 caggtccaaa gcttggcctc ccagcagccg ctgccagcct cagcagcccc tagaacaaac
661 actgtgacct cacaggtcca gcaggtccca gttgtactgc agccacactt catcaaggca
721 gactcactgc tgctgacagc tgtgaagaca gatgcaggag ccaccgtgaa gactgcaggc
781 atcagcaccc tggctcctgg cacagccgtg caggcaggtc ccctgcagac cctggtgagt
841 ggagggacca tcttggccac agtacctttg gttgtggaca cagacaaact gcccatccac
901 cgactcgcag ctggcagcaa ggccctaggc tcagctcaga gccgtggtga gaagcgcaca
961 gcccacaatg ccattgagaa gcgctaccgg tcttctatca atgacaagat tgtggagctc
1021 aaagacctgg tggtgggcac tgaagcaaag ctgaataaat ctgctgtctt gcgcaaggcc
1081 atcgactaca tccgcttctt gcagcacagc aaccagaagc tcaagcagga gaacctgacc
1141 ctacgaagtg cacacaaaag caaatcactg aaggacctgg tgtcagcttg tggcagtgga
1201 ggaggcacag atgtgtctat ggagggcatg aaacccgaag tggtggagac gcttacccct
1261 ccaccctcag acgccggctc accctcccag agtagcccct tgtcttttgg cagcagagct
1321 agcagcagtg gtggtagtga ctctgagccc gacagtccag cctttgagga tagccaggtc
1381 aaagcccagc ggctgccttc acacagccga ggcatgctgg accgctcccg cctggccctg
1441 tgtgtactgg cctttctgtg tctgacctgc aatcctttgg cctcgctttt cggctggggc
1501 attctcactc cctctgatgc tacgggtaca caccgtagtt ctgggcgcag catgctggag
1561 gcagagagca gagatggctc taattggacc cagtggttgc tgccacccct agtctggctg
1621 gccaatggac tactagtgtt ggcctgcttg gctcttctct ttgtctatgg ggaacctgtg
1681 actaggccac actctggccc ggctgtacac ttctggagac atcgcaaaca agctgacctg
1741 gatttggccc ggggagattt cccccaggct gctcaacagc tgtggctggc cctgcaagcg
1801 ctgggccggc ccctgcccac ctcaaacctg gatctggcct gcagtctgct ttggaacctc
1861 atccgccacc tgctccagcg tctctgggtg ggccgctggc tggcaggcca ggccgggggc
1921 ctgctgaggg accgtgggct gaggaaggat gcccgtgcca gtgcccggga tgcggctgtt
1981 gtctaccata agctgcacca gctgcatgcc atgggcaagt acacaggagg acatcttgct
2041 gcttctaacc tggcactaag tgccctcaac ctggctgagt gcgcaggaga tgctatctcc
2101 atggcaacac tggcagagat ctatgtggca gcggccctga gggtcaaaac cagcctccca
2161 agagccctgc acttcttgac acgtttcttc ctgagcagcg cccgccaggc ctgcctagca
2221 cagagcggct cggtgcctct tgccatgcag tggctctgcc accctgtagg tcaccgtttc
2281 tttgtggacg gggactgggc cgtgcacggt gcccccccgg agagcctgta cagcgtggct
2341 gggaacccag tggatccgct ggcccaggtg acccggctat tccgtgaaca tctcctagag
2401 cgagcgttga actgtattgc tcagcccagc ccaggggcag ctgacggaga cagggagttc
2461 tcagatgccc ttggatatct gcagttgcta aatagctgtt ctgatgctgc cggggctcct
2521 gcttgcagtt tctctgtcag ctccagcatg gctgccacca ctggcccaga cccagtggcc
2581 aagtggtggg cctcactgac agctgtggtg atccactggc tgaggcggga tgaagaggca
2641 gctgagcgct tgtacccact ggtagagcat atcccccagg tgctgcagga cactgagaga
2701 cccctgccca gggcagctct gtactccttc aaggctgccc gggctctgct ggaccacaga
2761 aaggtggaat ctagcccagc cagcctggcc atctgtgaga aggccagtgg gtacctgcgg
2821 gacagcttag cctctacacc aactggcagt tccattgaca aggccatgca gctgctcctg
2881 tgtgatctac ttcttgtggc ccgtaccagt ctgtggcagc ggcagcagtc accagcttca
2941 gtccaggtag ctcacggtac cagcaatgga ccccaggcct ctgctctgga gctgcgtggt
3001 ttccaacatg acctgagcag cctgcggcgg ttggcacaga gcttccggcc tgctatgagg
3061 agggtattcc tacatgaggc cacagctcgg ctgatggcag gagcaagtcc tgcccggaca
3121 caccagctcc tggatcgcag tctgaggagg agggcaggtt ccagtggcaa aggaggcact
3181 acagctgagc tggagccacg gcccacatgg cgggagcaca ccgaggccct gctgttggca
3241 tcctgctatc tgccccctgc cttcctgtcg gctcctgggc agcgaatgag catgctggcc
3301 gaggcggcac gcaccgtaga gaagcttggc gatcaccggc tactgctgga ctgccagcag
3361 atgctcctgc gcctgggcgg cggaaccacc gtcacttcca gctag |
