| Primer Pair Descriptions: |
| PrimerBank ID |
10946612a1 |
Validation Results (Click here to view experimental validation data: amplification plots, dissociation curves, 2% agarose gel analysis, sequencing and BLAST data) |
| Amplicon Size |
191 |
|
Sequence (5' -> 3') |
Length |
Tm |
Location |
| Forward Primer |
CAGCAACCTGGGTACATCCC |
20 |
62.3 |
82-101 |
| Reverse Primer |
CCAAGGTCATGGAAGTCTCGG |
21 |
62.1 |
272-252 |
| Location in Coding Sequence (primers and amplicon highlighted) |
1 atgactggcc gagcccgagc tcgggcccgc ggcagggcac gaggtcagga gacggtgcag
61 catgttgggg ctgctgcgag ccagcaacct gggtacatcc caccgagacc tcaacagtcc
121 cccacagagg gggacttggt tggccgagga cgacagaggg ggatggtagt cggagccaca
181 tccaagtcac aagaactgca gatctcagct gggtttcagg agctgtcact ggcagagaga
241 ggagggcgtc gccgagactt ccatgacctt ggtgtgaaca ccagacagaa ccttgaccat
301 gtcaaagagt caaagacagg ctcctctggc atcattgtga agctgagcac caaccacttc
361 cggctgacct cgcgcccaca gtgggccctg tatcagtacc acatcgacta caatcccctg
421 atggaggccc gaaggcttcg ctccgcactg ctcttccagc atgaagacct cattggaagg
481 tgtcatgctt tcgatgggac aatattgttt ttacctaaga gactacagca caaggtcaca
541 gaagtattca gtcagactcg gaatggggaa cacgtgagga tcaccatcac cctgaccaac
601 gagctgccgc ccacctcgcc cacctgcctg cagttctata acatcatctt caggaggctc
661 ttgaaaatca tgaatttgca acaaattgga cggaattatt acaatccaag tgacccgatt
721 gatattccaa accacaggtt ggtgatctgg cccggcttca ccacctccat ccttcagtat
781 gagaacaaca tcatgctctg cacagacgtc agccacaagg tgctccgcag cgagactgtc
841 ctagacttca tgttcaatct ataccagcag acagaggagc acaagttcca ggagcaagtg
901 tcgaaggagc tcataggcct catcgttctc accaagtaca ataacaagac ctaccgggtg
961 gatgacattg actgggacca gaatccaaag agcaccttca agaaggcgga tggctcggag
1021 gtcagcttcc tggagtacta caggaagcaa tacaaccagg agatcacgga cctgaagcag
1081 ccggtgctgg tgagccaacc caagcggagg agaggccccg gcggcaccct gcctggccca
1141 gctatgctca tccctgaact ctgctatctc acaggcctga ctgataaaat gcgcaatgat
1201 ttcaatgtga tgaaggacct ggcagtgcac acgcggctga cccctgagca gcggcagcgg
1261 gaggtgggcc gcctcatcga ctacatccac aaggatgaca atgtgcagag agagcttcga
1321 gactggggcc tgagcttcga ctcaaacttg ctgtccttct ctggaagaat cttacaatct
1381 gagaagatcc accagggcgg aaagacgttt gattacaacc cacaatttgc agactggtcc
1441 aaagaaacaa gaggcgcgcc gctgatcagc gtgaagccat tggataactg gctgctgatc
1501 tatacccgca ggaattatga agcagccaac tcactgatac agaacctgtt caaagtgact
1561 ccagccatgg gcatccagat gaaaaaggca atcatgatcg aggtggatga cagaacagaa
1621 gcttatctga gagccttgca gcagaaggtg acgtcagaca ctcagatagt tgtctgtctc
1681 ttgtcaagta atcggaagga caaatatgat gccatcaaga agtacttgtg tacagactgc
1741 cccaccccaa gtcagtgtgt ggtggcccgg accctgggca agcagcaaac agtcatggcc
1801 attgccacca agatcgccct gcagatgaac tgcaagatgg gaggcgagct ctggcgggtg
1861 gacatgcccc tgaaactggc aatgatcgtg ggcatcgact gttaccatga caccacagct
1921 gggcggaggt ccatcgcagg attcgtcgcc agcatcaatg aagggatgac ccgctggttc
1981 tcccgctgcg tctttcagga ccgcgggcag gagctggtgg atggtctcaa ggtgtgcttg
2041 caagctgctc tgagggcttg gagtggctgc aatgaataca tgcccagccg tgtcatcgtg
2101 taccgagacg gtgtggggga cgggcagctg aagaccctgg tcaattatga ggtcccacag
2161 ttcctagatt gcctcaagtc agtcgggaga ggttacaacc caagactgac tgtaatcgtg
2221 gtgaagaagc gtgtcaatgc caggtttttt gctcagtctg ggggaagact tcagaaccct
2281 cttccaggga cagtcatcga tgtggaagtc accagaccag agtggtatga ctttttcatc
2341 gtgagccagg cagtgagaag cgggagtgtg tccccaacac actacaatgt catctatgac
2401 agcagtggcc tgaagcccga ccacatccag cggctgacat acaagctctg ccacgtgtac
2461 tataattggc ctggagtcat ccgagtccct gcaccttgcc agtatgcaca caagctggcc
2521 ttcctcgtgg gccagagcat ccacagagag ccaaacctct ccctgtccaa ccgcctctac
2581 tacctctaa |
