Figure 5


Transposon Mating constructs used to create PA14 mutants: Two different transposons are used to create PA14 mutants for the non-redundant PA14 mutant library, Tn5-based TnphoA and mariner-based, MAR2xT7. Note that the TnPhoA transposon is marked with a Kanamycin/Neomycin resistance gene, whereas the Mar2xT7 transpson will confer Gentamycin resistance. Below each construct schematic is a depiction of the transposon inserted into the PA14 genome.
Figure 5 - Transposon Mating constructs used to create PA14 mutants: Two different transposons are used to create PA14 mutants for the non-redundant PA14 mutant library, Tn5-based TnphoA and mariner-based, MAR2xT7. Note that the TnPhoA transposon is marked with a Kanamycin/Neomycin resistance gene, whereas the Mar2xT7 transpson will confer Gentamycin resistance. Below each construct schematic is a depiction of the transposon inserted into the PA14 genome.