Figure 4


Arbitrary PCR Methodology: PCR Reaction 1 with primers 1 and ARB1 amplifies genomic DNA adjacent to the transposon. PCR Reaction 2 with nested primer 2 and ARB2 specifically enriches for the desired products from PCR Reaction 1. Products from PCR Reaction 2 are sequenced directly using primer 3.
Figure 4 - Arbitrary PCR Methodology: PCR Reaction 1 with primers 1 and ARB1 amplifies genomic DNA adjacent to the transposon. PCR Reaction 2 with nested primer 2 and ARB2 specifically enriches for the desired products from PCR Reaction 1. Products from PCR Reaction 2 are sequenced directly using primer 3.